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The Condensed Protocols From Molecular Cloning
The Condensed Protocols From Molecular Cloning: A Laboratory Manual is a single–volume adaptation of the three–volume third edition of Molecular Cloning: A Laboratory Manual. This condensed book contains only the step–by–step portions of the protocols, accompanied by selected appendices from the world’s best–selling manual of molecular biology techniques. 
Each protocol is cross–referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning.
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1. Using Plasmid Vectors in Molecular Cloning
2. Bacteriophage ? and Its Vectors
3. Working with Bacteriophage M13 Vectors
4. Working with High–capacity Vectors
5. Gel Electrophoresis of DNA and Pulsed–field Agarose Gel Electrophoresis
6. Preparation and Analysis of Eukaryotic Genomic DNA
7. Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells
8. In Vitro Amplification of DNA by the Polymerase Chain Reaction
9. Preparation of Radiolabeled DNA and RNA Probes
10. Working with Synthetic Oligonucleotide Probes
11. Preparation of cDNA Libraries and Gene Identification
12. DNA Sequencing
13. Mutagenesis
14. Screening Expression Libraries
15. Expression of Cloned Genes in Escherichia coli
16. Introducing Cloned Genes into Cultured Mammalian Cells
17. Analysis of Gene Expression in Mammalian Cells
18. Protein Interaction Technologies
By Prija Francis
MOLECULAR CLONING
Molecular cloning refers to the procedure of isolating a defined DNA sequence and obtaining multiple copies of it in vivo. Cloning is frequently employed to amplify DNA fragments containing genes, but it can be used to amplify any DNA sequence such as promoters, non-coding sequences and randomly fragmented DNA. It is utilised in a wide array of biological experiments and practical applications such as large scale protein production. Occasionally, the term cloning is misleadingly used to refer to the identification of the chromosomal location of a gene associated with a particular phenotype of interest, such as in positional cloning. In practice, localization of the gene to a chromosome or genomic region does not necessarily enable one to isolate or amplify the relevant genomic sequence.
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